GelstainRed 10000x in water

GelstainRedTM is a highly sensitive, stable, non-toxic, nonmutagenic fluorescent nucleic acid dye (working concentration). The spectra of GelstainRedTM is the same as EtBr, so it can replace EtBr without changing imaging system. In addition, GelstainRedTM is far more sensitive than EtBr.

Storage: Store at room temperature.

Protocol

1. Pre-cast Protocol for Agarose Gels (same as EB)

1. Add 5 μL of GelstainRedTM nucleic acid dye to every 50 mL of agarose gel during gel preparation and mix well. (GelstainRedTM has excellent thermal stability, and the reagent can be added directly to the high temperature gel solution without waiting for the gel solution to cool before adding. It can also be prepared by premixing GelstainRedTM with electrophoresis buffer containing agarose powder and heating).
   
2. Load samples and run gels according to your standard protocol.

2. Post-Stain Protocol

1. Run gel as usual according to your standard protocol.
   
2. Dilute GelstainRedTM 10,000× stock solution approximately 3,300 times into H2O to make 3× staining solution. (i.e., add 15 μL 10,000× GelstainRedTM to 50 mL H2O).
3. Carefully put the gel into a suitable container, and add enough 3× staining solution to immerse the gel. In order to shorten the soaking time, the staining solution can be pre-heated to about 70 °C, then put into the gel and incubated for 10 min. for ideal results. (If not heated, incubate at room temperature for 30 min on a shaker. If it is an acrylamide gel, incubate for 30 min to 1 h, and it will increase with the increase of acrylamide content.). The amount of foam dyeing dye is large, and the single-use dyeing solution can be reused about 3 times. 3×GelstainRedTM staining solution can be prepared in large quantities and stored at room temperature until used up.
4. Note:
   
1. If you always see the bands spread out or not well separated, it is recommended to use the bubble dye method to confirm whether the problem is related to the dye.
2. If the problem persists after soaking, it means that the problem is not related to the dye, please try: reduce the agarose concentration, choose a longer gel, extend the gel time to ensure sharp edges or improve the sample loading technique.